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Bioprospecting thermophiles for cellulase production: a review BJM
Acharya,Somen; Chaudhary,Anita.
Most of the potential bioprospecting is currently related to the study of the extremophiles and their potential use in industrial processes. Recently microbial cellulases find applications in various industries and constitute a major group of industrial enzymes. Considerable amount of work has been done on microbial cellulases, especially with resurgence of interest in biomass ethanol production employing cellulases and use of cellulases in textile and paper industry. Most efficient method of lignocellulosic biomass hydrolysis is through enzymatic saccharification using cellulases. Significant information has also been gained about the physiology of thermophilic cellulases producers and process development for enzyme production and biomass...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lignocelluloses; Bioethanol; Cellulase; Thermophiles.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300001
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Characterization of a thermostable endoglucanase produced by Isoptericola variabilis sp. IDAH9 BJM
Azizi,Maryam; Hemmat,Jafar; Seifati,Seyed Morteza; Torktaz,Ibrahim; Karimi,Soodabeh.
Abstract This study aimed to isolate and evaluate the cellulase activity of cellulolytic bacteria in hot springs of Dehloran, Ilam province, Iran. Water and sludge samples were collected from the hot springs and the bacterial enrichment was performed in a medium containing rice barn and carboxymethyl cellulose (CMC). The cultures were incubated at 50 °C in aerobic conditions. The bacteria were isolated on CMC agar (1%) medium. Cellulase assay of the isolates was measured by the evaluation of endoglucanase enzyme activity, which is also called as carboxymethyl cellulase (CMCase). The isolated thermotolerant bacteria were then identified and optimized for the production of CMCase. Moreover, stabilizing elements of the enzyme were identified with in silico...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carboxymethyl cellulase; Endoglucanase; Thermophiles; Protein structure.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401225
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Continuous enrichment cultures: insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys ArchiMer
Postec, Anne; Lesongeur, Francoise; Pignet, Patricia; Ollivier, B; Querellou, Joel; Godfroy, Anne.
The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga,...
Tipo: Text Palavras-chave: Metabolic interactions; 16S rRNA gene; Thermophiles; Bioreactor; Continuous enrichment cultures; Deep sea hydrothermal vent; Microbial diversity.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3885.pdf
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Cycles biogéochimiques du Fer et du Soufre dans les systèmes hydrothermaux en contexte sédimentaire du Bassin de Guaymas : traçages isotopiques et interactions micro-organismes/minéraux ArchiMer
Callac, Nolwenn.
Active hydrothermal chimney and sediments of the Guaymas Basin (Mexico) host various microbial communities with different metabolisms, including those involved in biogeochemical cycles of sulfur and iron. It is established that, in these dynamic ecosystems, microbial activity depends on the availability of substrates in their environment and that prokaryotes could, in return locally affect the composition of the hydrothermal fluid and mineralogical composition of the chimney or sediment, by mediating the dissolution and / or precipitation of some mineral phases. In order to study these prokaryotes-biotope interactions, and establish links between the structure, the activity and the isotopic signatures of microbial communities with the physico-chemical...
Tipo: Text Palavras-chave: Interactions procaryotes-biotopes; Cycle du fer; Cycle du soufre; Thermophiles; Cultures en bioréacteurs; Cultures en fioles; Module de colonisation in situ; Isotopie du fer; Isotopie du soufre; Systèmes hydrothermaux du Bassin de Guaymas; Prokaryote-biotope interactions; Iron cycle; Sulfur cycle; Thermophilic micro-organisms; Cultures in bioreactors; Cultures in batch; In situ colonization module; Iron isotope; Sulfur isotope; Hydrothermal systems of Guaymas Basin.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00498/60953/64356.pdf
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Desulfurobacterium atlanticum sp nov., Desulfurobacterium pacificum sp nov and Thermovibrio guaymasensis sp nov., three thermophilic members of the Desulfurobacteriaceae fam. nov., a deep branching lineage within the Bacteria ArchiMer
L' Haridon, S; Reysenbach, A.l.; Tindall, B. J.; Schonheit, P; Banta, A; Johnsen, U; Schumann, P; Gambacorta, A; Stackebrandt, E; Jeanthon, C.
Three thermophilic, anaerobic, strictly chemolithoautotrophic, sulphur- and/or thiosulphate-reducing bacteria, designated SL17(T), SL19(T) and SL22(T), were isolated from deep-sea hydrothermal samples collected at 13 degrees N (East Pacific Rise), Guaymas Basin (Gulf of California) and 23 degrees N (Mid-Atlantic Ridge), respectively. These strains differed in their morphology, temperature range and optimum for growth, energy substrates and 16S rRNA gene sequences. The G + C content of the genomic DNA was 41 mol% (SL22(T)), 42 mol% (SL17(T)) and 46 mol% (SL19(T)). Comparative analysis of phenotypic and phylogenetic traits indicated that strains SL17(T) and SL22(T) represented two novel species of the genus Desulfurobacterium and that strain SL19(T) should...
Tipo: Text Palavras-chave: Hydrothermal environment; Phylogeny; Thermophiles; Bacteria.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2174.pdf
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Optimization of fermentation conditions for cellulases production by Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from Indian hot spring BABT
Acharya,Somen; Chaudhary,Anita.
The aim of this work was to study the effect of some nutritional and environmental factors on the production of cellulases, in particular endoglucanase (CMCase) and exoglucanases (FPase) from Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from an Indian hot spring. The characterization study indicated that the optimum pH and temperature value was 6.5 to 7.0 and 50-55°C, respectively. Maximum cellulases production by both the isolates was detected after 60 h incubation period using wheat and rice straw. The combination of inorganic and organic nitrogen source was suitable for cellulases production. Overall, FPase production was much higher than CMCase production by both of the strains. Between the two thermophiles, the cellulolytic activity was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolation; Thermophiles; Submerged fermentation; Parameter optimization; Cellulases; Hot spring.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400003
Registros recuperados: 6
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